Development and Standardization of a Liposomal 4-Thiourendiminomethylpyridinium Perchlorate Using Natural Triacylglycerols // Nature of Inner Asia. - 2026. №1(34). . - С. 94-105.

Development and Standardization of a Liposomal 4-Thiourendiminomethylpyridinium Perchlorate Using Natural Triacylglycerols

DOI: 10.18101/2542-0623-2026-1-94-105 UDK: 615.451.3:547.915:665.3

Development of new dosage forms of anti-tuberculosis drugs is a pressing issue for regions with a high incidence of this disease, including the Republic of Buryatia. The article presents the results of the development of 4-thiourendoiminomethylpyridinium perchlorate (Perchlozon) liposomal formulation, characterized by lipophilicity and limited bioavailability. We used natural triacylglycerols — Siberian marmot (Marmota sibirica Radde) fat and pine nut (Pinus sibirica) pellicle extract — to modify the lipid bilayer. The fatty acid composition of the starting materials was determined using GC-MS: 36 fatty acids were identified in marmot subcutaneous fat and 20 — in pine nut membrane extract. We obtained a polyunsaturated fatty acid concentrate from marmot subcutaneous fat via complexation with urea; the linolenic acid content reached 45.3 % and the linoleic acid content — 21.7 %. The technology for producing liposomes via thin film hydration was optimized: optimal ultrasonic treatment time ensuring a drug incorporation rate of 56.5% was 7 min with an average vesicle size of 445±55 nm. We developed methods for standardizing the finished product using UV spectrophotometry (analytical wavelength — 317 nm) and HPLC, allowing for confirmation of authenticity and determination of the active ingredient content. The results obtained serve as the basis for the draft corporate pharmacopoeial monograph.

liposomes, liposomal delivery, «Perchlozon», Siberian marmot fat, pine nuts, polyunsaturated fatty acids, standardization.