BSU bulletin. Medicine and pharmacy
Bibliographic description:
, , DEVELOPMENT OF A METHOD FOR QUANTITATION OF THE TOTAL FLAVONOIDS CONTENT IN ARTEMISIA SUBVISCOSAE HERBA // BSU bulletin. Medicine and pharmacy. - 2021. №1. . - С. 52-57.
Title:
DEVELOPMENT OF A METHOD FOR QUANTITATION OF THE TOTAL FLAVONOIDS CONTENT IN ARTEMISIA SUBVISCOSAE HERBA
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Annotation:
The article presents a method for quantitation of the total flavonoids content in the aerial part of Artemisia subviscosa by UV-spectrophotometry. We have collected full spec- tral data on alcoholic extraction in a complex with aluminum chloride; the absorption max- imum at a wavelength of 400 nm of the test solution corresponds to the absorption maxi- mum of the spectrum of luteolin-7-glycoside with aluminum chloride. We have determined the optimal extraction conditions: type of extractant — 40% ethyl alcohol, grinding degree of raw materials — 1 mm, ratio of raw materials to extractant — 1:50, temperature — boil- ing water bath, extraction time — 30 min, twice. Aluminum chloride was used as a com- plexing agent (2 ml of a 2% solution); the complex was formed within 45 minutes. The total flavonoids content equivalent to luteolin-7-glycoside in A. subviscosa herba is 2.03 ± 0.04%. The relative error of the proposed method does not exceed 5%.
Keywords:
Asteraceae, Artemisia L., Artemisia subviscosa, phenolic compounds, flavonoids
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Article.pdf