, , REGENARATION OF LARIX SIBIRICA LEDEB. IN IN VITRO CULTURE THROUGH THE SOMATIC EMBRYOGENESIS AND THE EFFECT OF MYCORHYZA ON ITS GROWTH IN SOIL // Nature of Inner Asia. - 2020. №3/4(16). . - С. 77-83.

REGENARATION OF LARIX SIBIRICA LEDEB. IN IN VITRO CULTURE THROUGH THE SOMATIC EMBRYOGENESIS AND THE EFFECT OF MYCORHYZA ON ITS GROWTH IN SOIL

DOI: 10.18101/2542-0623-2020-3/4-77-83 UDK: 58.085

The article presents the results of the analysis of Larix sibirica Ledeb. reproduction in in vitro culture through the somatic embryogenesis and the effect of mycorrhiza on their growth in the soil environment. To obtain microclonal Sibe- rian larch it is effective to use 1 mg / L 2,4-D (2,4-dichlorophenoxyacetic acid) and 2 mg / L 6-BAP (6-benzylaminoperemedin) as growth regulators with pH-5.8. The dosage for preparation of mycorrhiza according to the manufacturer’s instructions was 3.25 ml with the addition of 15 ml of water. In microclonal plants that have been infect- ed with mycorrhiza, lateral roots are formed from the main roots of the plants, and the average number of branched roots in 15 plants is 8.8 cm.The average length of the main root is 6.7 cm and 3.4 cm longer than in plants that have not been infect- ed with mycorrhiza. The survival rate of microclonal plants is 64. Thus, mycorrhi- zae increase the amount of water and nutrients absorbed by the plant root and create a connection between roots and soil, branching the plant roots over a wider area.

in vitro; MSGM nutrient medium; somatic embryogenesis; Siberian larch; micro- clonal plants; mycorrhiza.