, , , , , , Developing Biopolymeric Scaffolds for Regenerative Tissue Genesis // BSU bulletin. Medicine and pharmacy. - 2017. №3. . - С. 47-52.

Developing Biopolymeric Scaffolds for Regenerative Tissue Genesis

Работа выполнена при поддержке грантов для проведения инновационных исследований БГУ и РФФИ № 16-44-030854

UDK: 615,468: 576,54

Development and introduction of new biotechnological analogues of tissues and organs into clinical practice, in particular human tissue-engineered skin equivalents, designed for temporal or permanent replacement of damaged or destroyed tissue, remains an ur- gent problem of modern medicine. One of the most promising approaches for tissue re- constitution is an application of bioderived materials such as biodegradable 3D matrix- es. Combining collagen extraction, gelation of collagen as flat matrixes, culturing Ha- CaT keratinocytes on the formed matrixes with subsequent fixation and decellulariza- tion allowed to develop nonliving acellular dermo-epidermal skin analogues consisting of two main components –collagen and laminin. The in vivo evaluation of the skin scaffolds transplanted onto full-thickness skin wounds created on the back of rats showed enhanced wound healing. a new protein scaffold consisting of two components — collagen and laminin of basal membrane mimicking the structure of human skin is proposed for implementation in regenerative medicine.

regenerative medicine; tissue engineering; wound healing; matrix; tissue- engineered skin; skin equivalents; biopolymers; cell culture; keratinocytes; collagen; laminin.